| 菌株簡介 |
E. coli S17-1 λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific host strain into which the transposon vector DNA is transformed, the transfer occurs by biparental mating (a helper strain is not necessary).E. coli S17-1 λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific host strain into which the transposon vector DNA is transformed, the transfer occurs by biparental mating (a helper strain is not necessary). The genotype of the strain is TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: Km Tn7 λpir. |
