Fluorescence detection
Fluorescence imaging
FACS
Cell infection assays In vivo localization
Pathogen-host interactions assays
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Type Strain
no
Antibiotic Resistance
Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin.
Comments
This clone was derived from ATCC® 10145™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.
Vector: pUCP18-MCSgfpmut3 Vector size: 5.492kb Vector type: plasmid Marker:bla (ampR) Features: GFPmut3: fluorescent maker under the control of Plac promoter Origin of replication: ori from pRO1600 Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI Double restriction sites: HindIII, PstI, SalI, XbaI
Medium
ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin
Growth Conditions
Temperature: 37°C Atmosphere: Aerobic
Name of Depositor
M Barbier
Chain of Custody
Modified by ATCC, Dr. M. Barbier <-- ATCC <-- F Kavanagh <-- Merck Sharp & Dohme
References
Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.
Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227
Opinion 36. Designation of strain ATCC 10145 as the neotype strain of Pseudomonas aeruginosa (Schroeter) Migula. Int. J. Syst. Bacteriol. 20: 15-16, 1970.
Hugh R, Leifson E. The proposed Neotype Strains of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900. Int. Bull. Bacteriol. Nomencl. Taxon 14: 69-84, 1964.
