| 產品名稱 |
pCW8 plasmid in E. coli |
| 商品貨號 |
B161818 |
| Designations |
pCW8 plasmid in E. coli |
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
R. Kopito |
| Vector |
Construct size (kb): 4.136 Vector: pRBG4 Vector size (kb): 3.806 |
| Insert |
Ubiquitin, mutant Insert size (kb): 0.330 (Ubiquitin, 6HIS and c-myc tag) Insert site: ClaI/EcoRI Excise insert: EcoRI |
| Putative ID |
ubiquitin |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
This is a cDNA clone of ubiquitin K48R from Saccharomyces cerevisiae with a 6 HIS, c-myc N-terminal tag cloned into the ClaI/EcoRI sites of the pRBG4 vector. The plasmid was transformed into E. coli DH10B. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
Ward CL, et al. Degradation of CFTR by the ubiquitin-proteasome pathway. Cell 83: 121-127, 1995.
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