| Insert |
DNA: genomic Insert lengths(kb): 2.799999952316284 Tissue: DNA from rodent/human hybrid cell line (6p only) Gene product: DNA Segment, single copy [D6S90] Alleles: A1, A2, B2, B1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.8; BamHI--3.8, 2.0; SalI--5.8; PstI--5.8; HindIII--5.8. The depositor has requested that the HGM regional assignment be changed to 6p12-p21 on the human chromosome. Prehybridization with human placental DNA is required prior to hybridization at normal stringency (65C, 1 M Na+). Enzyme(s) not detecting polymorphism: ApaI, BamHI, BanI, BanII, BglI, BglII, DraI, EcoRI, EcoRV, Fnu4HI, HaeIII, HindIII, HinfII, MboI, PstI, PvuII, RsaI, ScaI, SphI, SstI, TaqI, XbaI. |
| References |
Huang LS, et al. BclI and MspI polymorphisms at the D6S90 locus. Nucleic Acids Res. 18: 5922, 1990. PubMed: 1699208
Huda Y Zoghbi, personal communication
|
