| 產品名稱 | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品貨號 | B161975 |
| Deposited As | Clostridium difficile (Hall and O'Toole) Prevot |
| Strain Designations | 90556-M6S |
| Application | Produces cytotoxin
Enteric Research
|
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Preceptrol® | no |
| Genotype | Toxinotype: 0 |
| Comments | Presence of tcdA and tcdB genes confirmed by PCR. Ref
 Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001
|
| Medium | ATCC® Medium 2107: Modified Reinforced Clostridial ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood |
| Growth Conditions | Temperature: 37°C Atmosphere: Anaerobic |
| Name of Depositor | FE Clark |
| Special Collection | NSF - Bacteriology |
| Chain of Custody | ATCC <-- F E Clark |
| References | Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634 Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 |
| Cross References | Nucleotide (GenBank) : X94328 C.difficile spo0A gene. Nucleotide (GenBank) : AX110003 Sequence 736 from Patent WO0123604. Nucleotide (GenBank) : AX110007 Sequence 740 from Patent WO0123604. Nucleotide (GenBank) : AX110095 Sequence 828 from Patent WO0123604. Nucleotide (GenBank) : AX111484 Sequence 2217 from Patent WO0123604. Nucleotide (GenBank) : AX111505 Sequence 2238 from Patent WO0123604. Nucleotide (GenBank) : AX111525 Sequence 2258 from Patent WO0123604. Nucleotide (GenBank) : U36481 Clostridium difficile sporulation protein homolog (spo0A) gene, partial cds. Nucleotide (GenBank) : S51968 {16S/23S ribosomal DNA spacer region} [Clostridium difficile, ATCC 9689, Genomic, 271 nt] Nucleotide (GenBank) : U43514 Clostridium difficile putative spoIVB (spoIVB) gene, complete cds, and phosphorylation-activated transcription factor spo0A (spo0a) gene, complete cds. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
