| 產品名稱 |
CIA-E-4.15 |
| 商品貨號 |
B162028 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with human epsilon heavy chain. |
| Derivation |
Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human epsilon immunoglobulin heavy chain (IgE) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane-primed BALB/c mice (Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice.) |
| Comments |
Animals were immunized with a human IgE paraprotein (PS). Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells. The antibody reacts with human epsilon heavy chain. It has a very high affinity (3 X 10 exp-10 m/L) for an epitope located between amino acids 218 and 301 (CH2 domain) of human epsilon. It does not bind to protein A, but functions well in histamine release assays. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
A Saxon |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Macy E, et al. Enhanced ELISA: how to measure less than 10 picograms of a specific protein (immunoglobulin) in less than 8 hours. FASEB J. 2: 3003-3009, 1988. PubMed: 3263291
Hassner A, Saxon A. Isotype-specific human suppressor T cells for IgE synthesis activated by IgE-anti-IgE immune complexes. J. Immunol. 132: 2844-2849, 1984. PubMed: 6233363
Generally, the first i.p. passage yields mostly adherent tumor cells and little ascites, but serial passage of the ascites cell in additional BALB/c mice will yield large amounts of ascites that may contain up to 20 mg/ml of the antibody. For monoclonal antibody production in ascites, cells should be injected (10 to 20 million cells) intraperitoneally (i.p.) in pristane-primed BALB/c mice.
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