Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Shipping Information
Shipped: ?Freeze dried E. coli
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Genotype
P2old+ mcrA delta(mcrBC-hsdRMS-mrr)102 supE supF
Type Strain
no
Comments
Not tolerant to sequences that form secondary structure. Permits Spi selection of recombinant phage
A P2 lysogen of Escherichia coli PMC104 (ATCC 47061)
High-efficiency, methylation-tolerant host for bacteriophage lambda with or without amber mutations. Not tolerant to sequences that form secondary structure. Permits Spi selection of recombinant phage.
This strain is being made readily available to the scientific community with the proviso that it not be used to form the basis of a product for commercial sale or be included in any commercial product.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor
JP Doherty
References
Doherty JP, et al. Escherichia coli host strains SURE (TM) and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains. Gene 124: 29-35, 1993. PubMed: 8440479
