| 產品名稱 | MB355 |
|---|---|
| 商品貨號 | B162100 |
| Organism | Mus musculus, mouse |
| Tissue | embryo |
| Cell Type | fibroblast, spontaneously immortalized |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 14.5 days gestation embryo |
| Applications | DNA repair studies |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | MB355 (Polb/p53 double null) is a mouse embryonic fibroblast (MEF) cell line derived from polymerase (DNA directed), beta (Polb)/p53 double null embryos at day 14.5 of gestation. The cells were spontaneously immortalized at passage 5 due to the loss of the p53 gene. The cells are transgenic for lambda LIZ (Lac I/cII). |
| Comments | MB355 (Polb/p53 double null) is a mouse embryonic fibroblast (MEF) cell line derived from polymerase (DNA directed), beta (Polb)/p53 double null embryos at day 14.5 of gestation. The cells were spontaneously immortalized at passage 5 due to the loss of the p53 gene. The cells are transgenic for lambda LIZ (Lac I/cII). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Protocol:
Interval: Maintain cultures at a cell concentration between 6 X 10(3) and 1 X 10(5) cells/cm2. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended Medium Renewal: Every two to three days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Population Doubling Time | 20 hours |
| Name of Depositor | RW Sobol |
| Passage History | MB355 (Polb/p53 double null) is a mouse embryonic fibroblast (MEF) cell line derived from polymerase (DNA directed), beta (Polb)/p53 double null embryos at day 14.5 of gestation. The cells were spontaneously immortalized at passage 5 due to the loss of the p53 gene. The cells are transgenic for lambda LIZ (Lac I/cII). |
| Year of Origin | 2000 |
| References | Sobol RW, et al. Requirement of mammalian DNA polymerase-beta in base-excision repair. Nature 379: 183-186, 1996. PubMed: 8538772 Sobol RW, et al. Base excision repair intermediates induce p53-independent cytotoxic and genotoxic responses. J. Biol. Chem. 278: 39951-39959, 2003. PubMed: 12882965 Sobol RW, et al. Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress. Proc. Natl. Acad. Sci. USA 99: 6860-6865, 2002. PubMed: 11983862 Jacks T, et al. Tumor spectrum analysis in p53-mutant mice. Curr. Biol. 4: 1-7, 1994. PubMed: 7922305 Sobol RW, et al. The lyase activity of the DNA repair protein beta-polymerase protects from DNA-damage-induced cytotoxicity. Nature 405: 807-810, 2000. PubMed: 10866204 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
