| 產(chǎn)品名稱 |
HS-Sultan |
| 商品貨號(hào) |
B162193 |
| Organism |
Homo sapiens, human |
| Cell Type |
B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, multicell aggregates |
| Biosafety Level |
2 [Cells contain EBV DNA viral sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Burkitt's lymphoma |
| Age |
7 |
| Gender |
male |
| Ethnicity |
Black, African |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This is a hyperdiploid human cell line. The modal chromosome number was 48, occurring in 80% of cells, and the rate of polyploid cells was 3.4%. Three marker chromosomes are common to all cells. M1 and M2 [der(14)t(8;14) (q24;q32)] were probably derived from the balanced translocation between del(8) (p21.1;p21.3) and N14. The third marker, M3, is ?der(8)t(8;?) (p21.1;?). Most cells had three copies of N7. Both normal X and Y were clearly identifiable. |
| Derivation |
Although deposited with the ATCC as a plasmacytoma cell line, DNA fingerprinting has shown this line to be a derivative of Jiyoye (ATCC CCL-87), a Burkitt's lymphoma cell line. |
| Clinical Data |
Black, African male 7 |
| Antigen Expression |
CD11a +; CD19 +; CD20 +; CD28 -; CD38 +; CD49e + |
| Genes Expressed |
immunoglobulin |
| Cellular Products |
immunoglobulin |
| Comments |
The cells are EBNA positive (EBNA +). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 105 viable cells/mL. Maintain cell density between 3 X 105 and 3 X 106 viable cells/mL. Do not allow the cell density to exceed 4 X 106 cells/mL.
Medium Renewal: Every 3 to 4 days |
| Cryopreservation |
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,11 D13S317: 12 D16S539: 10,11 D5S818: 12 D7S820: 8,10 THO1: 7,9 TPOX: 6,8 vWA: 15,19 |
| Isotype |
kappa light chain |
| Name of Depositor |
NS Harris |
| Deposited As |
Homo sapiens |
| References |
Harris NS. Plasma cell surface antigen on human blood lymphocytes. Nature 250: 507-509, 1974. PubMed: 4549735
. . J. Immunol. 118: 1480-1482, 1976.
. . Proceedings of the Seventh Leukocyte Culture Conference : 15-28, 1973.
Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
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