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COLO 829BL

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編號(hào):B162220

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產(chǎn)品名稱	COLO 829BL
商品貨號(hào)	B162220
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	2 [Cells contain Herpesvirus] Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	normal
Age	45 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase
Derivation	The specimen was taken from a patient prior to chemotherapy for metastatic melanoma. The COLO 829BL cell line was established at the ATCC by Epstein-Barr virus (EBV) transformation of of a sample of peripheral blood provided by G.E. Moore. A cell line was also produced from the melanoma, and is available as COLO 829 (ATCC CRL-1974).
Clinical Data	45 years male Caucasian
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4 x 10⁵ viable cells/mL. Maintain cell density between 2 x 10⁵ and 2 x 10⁶ viable cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC^? Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Name of Depositor	GE Moore
Deposited As	Homo sapiens
References	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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