Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 2.4; EcoRI--6.4; XbaI--6.4. Care should be taken when cloning into this vector because several stop codons occur in this reading frame. One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter. Vectors YCpIF7, YCpIF8 and YCpIF9 (ATCC 87084-87086) differ only in the reading frame of the multiple cloning site. |
