| 產品名稱 |
SCC-15 |
| 商品貨號 |
B162570 |
| Organism |
Homo sapiens, human |
| Tissue |
tongue |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
squamous cell carcinoma |
| Age |
55 years |
| Gender |
male |
| Storage Conditions |
Liquid nitrogen vapor phase |
| Clinical Data |
male
55 years |
| Genes Expressed |
epidermal keratins; 40 kD keratin; low levels of involucrin |
| Cellular Products |
epidermal keratins; 40 kD keratin; low levels of involucrin |
| Tumorigenic |
Yes |
| Effects |
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments |
Unlike SCC-4 (ATCC CRL-1624), this line does not grow well in semi-solid medium, and does not require a feeder layer. |
| Complete Growth Medium |
A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,14 D16S539: 12,15 D5S818: 12 D7S820: 10,11 THO1: 9,9.3 TPOX: 8 vWA: 15,17 |
| Name of Depositor |
JG Rheinwald |
| Deposited As |
Homo sapiens |
| References |
Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336
Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916
Wu YJ, Rheinwald JG. A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas. Cell 25: 627-635, 1981. PubMed: 6169443
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