| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.3, 1.4; EcoRI--3.0, 2.7; XbaI--4.0, 1.7; SacI/XhoI--2.95, 1.25, 1.0, 0.5; HindIII--4.1, 1.6. The depositor has requested that the HGM regional assignment be changed to 6p12-p21 on the human chromosome. Prehybridization with human placental DNA is required prior to hybridization at normal stringency (65C, 1 M Na+). Enzymes not detecting polymorphism: ApaI, BamHI, BanI, BanII, BclI, BglI, BglII, DraI, EcoRI, EcoRV, Fnu4HI, HaeIII, HindIII, HinfII, MboI, PstI, PvuII, RsaI, ScaI, SstI, XbaI. |
