Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746
This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain.
cell enlargement, plaques with rounding and eventual sloughing of cells
Non-permissive incubation temperature is 39.5C. This mutant was assigned to Dales EM category F, indicating that it has aberrant membranes with spicules and viroplasm. It is also assigned the Condit map location G3. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane. This mutant was selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine.
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
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