| 產品名稱 |
Human herpesvirus 5 |
| 商品貨號 |
B162785 |
| Classification |
Herpesviridae, Cytomegalovirus |
| Agent |
Human herpesvirus 5 |
| Strain |
Merlin |
| Common Name |
Human cytomegalovirus |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Genome Sequenced Strain |
Human herpesvirus 5 strain Merlin, complete genome
|
| Comments |
Low MOI applications, such as titration, may require incubation for as long as 3 weeks. This is a low passage strain of Human herpesvirus 5, deposited at ATCC® 4 passages from isolation. At deposit, it retained the wild type complement of 165 genes, and contained no obvious mutations other than a single nucleotide substitution that truncates gene UL128. To retain genetic integrity, further passage should be limited. |
| Effect on Host |
CPE, cell rounding and enlargement, cytoplasmic and nuclear inclusions |
| Recommended Host |
MRC-5 (ATCC® CCL-171™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
|
| Name of Depositor |
GWG Wilkinson, Cardiff Public Health Laboratory |
| Source |
Urine from a congenitally infected infant, Cardiff, Wales |
| References |
Davison A J, et al. Homology between the human cytomegalovirus RL11 gene family and human adenovirus E3 genes. J. Gen. Virol. 84 (Pt 3), 657-663 (2003). PubMed: 12604818
Dolan A, et al. Genetic content of wild-type human cytomegalovirus. Journal of General Virology, 85: 1301-1312, 2004. PubMed: 15105547
|
| Cross References |
Nucleotide (GenBank) :
AY446894.2
Human herpesvirus 5 strain Merlin, complete genome
|
