| 產品名稱 |
Porcine rotavirus |
| 商品貨號 |
B162786 |
| Classification |
Reoviridae, Rotavirus |
| Agent |
Porcine rotavirus |
| Strain |
OSU (attenuated) |
| Applications |
Serotype 5 (G5) by antigenic typing at VP7 and serotype P7 by antigenic typing at VP4. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
clinical specimen - animal Ohio, United States |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder
(or colder) |
| Comments |
Serotype 5 (G5) by antigenic typing at VP7 and serotype P7 by antigenic typing at VP4. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated. |
| Effect on Host |
Yes, in vitro effects: Cytopathic effects, some initial rounding followed by degeneration of cells and eventual sloughing CPE, some initial rounding followed by ????? degeneration of cells and eventual sloughing |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle?s BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 0.008 mg/ml trypsin (GIBCO 27250-018). No serum is used.
|
| Recommended Host |
Recommended Host: MA-104 cells (ATCC CRL- ????? 2378.1) |
| Growth Conditions |
Temperature: 37.0°C Duration: 1-4 days; 5% CO2 in air atmosphere is recommended; For best results cells should be 24 to 48 hours old and 90-100% confluent. Protocol: This rotavirus should be grown in the presence of trypsin or pancreatin and NO serum should be used. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated, but no back passages have been done. Cross-reacts with group A rotaviruses from other species. Heat labile (56°C, 30min.). Stable at pH 3.0 and to ether. |
| Cryopreservation |
Storage temperature: -70°C |
| Subcultivation |
Protocol: This rotavirus should be grown in the presence of trypsin or pancreatin and NO serum should be used. Preliminary trials in gnotobiotic pigs indicate that this preparation is attenuated, but no back passages have been done. Cross-reacts with group A rotaviruses from other species. Heat labile (56°C, 30min.). Stable at pH 3.0 and to ether. |
| Name of Depositor |
LJ Saif |
| Source |
clinical specimen - animal Ohio, United States |
| References |
Saif LJ, et al. Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. Am. J. Vet. Res. 38: 13-20, 1977. PubMed: 189646
Theil KW, et al. Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. Am. J. Vet. Res. 39: 213-220, 1978. PubMed: 204232
Rosen BI, et al. Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. J. Clin. Microbiol. 30: 2644-2652, 1992. PubMed: 1328281
Theil KW, et al. Cell culture propagation of porcine rotavirus (reovirus-like agent). Am. J. Vet. Res. 38: 1765-1768, 1977. PubMed: 201198
or colder
intestinal contents of one- to four-week-old pigs with diarrhea
or colder
|
