| 產品名稱 | Human astrovirus |
|---|---|
| 商品貨號 | B162799 |
| Classification | Astroviridae, Mamastrovirus, Mamastrovirus 2 |
| Agent | Human astrovirus |
| Strain | Type 2 |
| Applications | Virucide testing In vitro infectivity assay Antiviral Studies Spiking studies ELISpot Assay Virus neutralization assay Virus ultrastructural studies Immunological-based assays PCR-based assay validation Research and development Assay development Vaccine development |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | Freeze-thaw twice at harvest. |
| Effect on Host | CPE may not be visible – confirm infection by IFA. Sloughing and detachment may result from trypsin supplemented growth medium. |
| Recommended Host | CaCo-2 cells (ATCC® HTB-37™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 90-100% confluent. Pre-treat inoculum in EMEM (ATCC® 30-2003™) + 5 μg/mL 1X-S trypsin (Sigma-Aldrich® T0303) for 30 minutes at 37°C. Remove medium from host cells and rinse twice with 1X DPBS. Inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (0.1 to 1.0). Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by removing inoculum and adding virus growth medium. Incubation: 1-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | J Vinje, CDC, Division of Viral Diseases |
| Source | Human stool sample, Oxford, UK |
| References | Lee TW, Kurtz JB. Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes. Epidemiol Infect. 1112(1): 187-193, 1994. PubMed: 8119358 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
