| 產品名稱 | Viral hemorrhagic septicemia virus |
|---|---|
| 商品貨號 | B162834 |
| Classification | Rhabdoviridae, Novirhabdovirus, Piscine novirhabdovirus |
| Agent | Viral hemorrhagic septicemia virus |
| Strain | Makah |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | Derived by adaptation of CHSE (ATCC® CRL-1681™) cell culture product ATCC® VR-1387™ to EPC cells (ATCC® CRL-2872™). ATCC® VR-1387™ and ATCC® VR-1872™ have not been compared for sequence or infectivity. |
| Effect on Host | CPE, refractile rounding and degeneration |
| Recommended Host | Epithelioma Papulosum Cyprini (EPC) cells (ATCC® CRL-2872™) |
| Growth Conditions | Temperature: 15°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 15°C in a humidified atmosphere. End adsorption by adding virus growth medium. Incubation: 2-4 days at 15°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | J Winton |
| Source | Isolated by R. Brunson from ovarian fluid of normal adult coho salmon returning in 1988 to the Makah National Fishery, WA. |
| References | Bernard J, et al. Nucleocapsid gene sequence of a North American isolate of viral haemorrhagic septicaemia virus, a fish rhabdovirus. J. Gen. Virol. 73: 1011-1014, 1992. PubMed: 1634868 Winton JR. . Proc. 2nd Int. Symp. Viruses Lower Verts : 43-50, 1991. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
