| 產(chǎn)品名稱 | 
      Human Coxsackievirus B4 | 
    
    
      | 商品貨號(hào) | 
      B162872 | 
    
      
      | Classification | 
      Picornaviridae, Enterovirus, Enterovirus B | 
    
      
      | Deposited As | 
      Coxsackievirus B 4 | 
    
      
      | Agent | 
      Human Coxsackievirus B4 | 
    
      
      | Strain | 
      J.V.B. (Benschoten) | 
    
      
      | Biosafety Level | 
      2 
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.   | 
    
      
      | Isolation | 
      Stool from 10-year-old child, New York, 1951  | 
    
      
      | Product Format | 
      frozen 1.0 mL | 
    
      
      | Storage Conditions | 
      -70°C or colder | 
    
      
      | Images | 
        | 
    
      
      | Effect on Host | 
      CPE, cell rounding and sloughing  | 
    
      
      | Recommended Host | 
      
LLC-MK2 Derivative (ATCC® CCL-7.1™)
  | 
    
      
      | Growth Conditions | 
      Temperature: 37°C 
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. 
Incubation: 
2-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. | 
    
      
      | Name of Depositor | 
      H Plager | 
    
      
      | Source | 
      Stool from 10-year-old child, New York, 1951 | 
    
      
      | Year of Origin | 
      1951 | 
    
      
      | References | 
      
            Dalldorf G. The Coxsackie viruses.  Bull NY Acad.. Med 26: 329-335, 1950. PubMed: 15411582
         
    
        
            Sickles GM, et al. Isolation and type determination of Coxackie virus, group B, in tissue culture.  Proc. Soc. Exp. Biol. Med. 88: 22-24, 1955. PubMed: 14357332
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