| 產品名稱 | 
      Murine hepatitis virus | 
    
    
      | 商品貨號 | 
      B162885 | 
    
      
      | Classification | 
      Nidovirales, Coronaviridae, Coronavirus,Group 2 | 
    
      
      | Deposited As | 
      Mouse hepatitis virus | 
    
      
      | Agent | 
      Murine hepatitis virus | 
    
      
      | Strain | 
      MHV-JHM | 
    
      
      | Common Name | 
      Murine hepatitis virus | 
    
      
      | Biosafety Level | 
      2 
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.   | 
    
      
      | Isolation | 
      tissue, animal  | 
    
      
      | Product Format | 
      frozen | 
    
      
      | Storage Conditions | 
      -70°C or colder
                                                
                                            (or colder) | 
    
      
      | Effect on Host | 
      Yes, in vitro effects: cytopathic effects in tissue culture, fusing and clumping of cells with lots of floating cells CPE, fusing and clumping cells, cells floating   | 
    
      
      | Recommended Host | 
      Recommended Host: NCTC clone 1469 cells (ATCC? CCL-9.1?) ??  | 
    
      
      | Growth Conditions | 
      
Atmosphere: 5% CO2 in air recommended 
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent). 
Temperature: 37°C 
  | 
    
      
      | Cryopreservation | 
      Storage temperature: -70°C  | 
    
      
      | Name of Depositor | 
      J Parker | 
    
      
      | Special Collection | 
      ATCC | 
    
      
      | Source | 
      tissue, animal  | 
    
      
      | References | 
      
            Cheever FS, et al. A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. J. Exp. Med. 90: 181-194, 1949. 
         
    
        
            or colder
         
    
        
            brain of 17-day-old Swiss mouse with spontaneous flaccid paralysis of hind legs
         
    
        
            or colder
          |