| 產品名稱 |
Human echovirus 9 |
| 商品貨號 |
B162945 |
| Classification |
Picornaviridae, Enterovirus, Enterovirus B |
| Deposited As |
Echovirus 9 |
| Agent |
Human echovirus 9 |
| Strain |
Hill |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Rectal swab from healthy child, Ohio |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder
(or colder) |
| Comments |
This strain is not pathogenic for newborn mice. Related antigenically to Coxsackievirus Type A23. Wigand, R. et al., Arch. Gesamte Virusforsch. 11: 708, 1962. |
| Effect on Host |
Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing) CPE, refractile rounding and sloughing |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host |
Recommended Host:? LLC-MK2 (ATCC CCL-7) |
| Growth Conditions |
Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent) for inoculation. |
| Cryopreservation |
Storage temperature: -70°C |
| Name of Depositor |
HA Wenner |
| Source |
Rectal swab from healthy child, Ohio |
| References |
Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336
Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578
Wigand R, Sabin AB. Antigenic purity and plaque properties of the prototype strains of ECHO virus types 7 to 11, and 17 and 18. Arch. Gesamte Virusforsch. 11: 708-717, 1962. PubMed: 14040255
or colder
or colder
|
| Cross References |
Nucleotide (GenBank) :
U11710
Echovirus 9 Hill polyprotein gene, 5' UTR.
|
