| 產品名稱 | Human adenovirus 42 |
|---|---|
| 商品貨號 | B162976 |
| Classification | Adenoviridae, Mastadenovirus, Human adenovirus D |
| Agent | Human adenovirus 42 |
| Strain | 54/82 |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | No cross-reactions by serum neutralization. Significant cross-reactions with Human adenovirus 15 and 22, bilaterally, by hemagglutinin inhibition assay. Hemagglutinates human or rhesus red blood cells. VR-1792™ was derived from VR-1304™ following treatment with diethyl ether to remove mycoplasma contamination. |
| Effect on Host | CPE, refractile rounding and sloughing |
| Recommended Host | HEp-2 (ATCC® CCL-23™) |
| Growth Conditions | Temperature: 36°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.05). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-4 days |
| Name of Depositor | JC Hierholzer, Centers for Disease Control and Prevention; ATCC |
| Source | Stool from an asymptomatic 1 year old male, Paris, 1982. Treated to remove mycoplasma at ATCC. |
| References | Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Wigand R, et al. A new human adenovirus of subgenus D: candidate adenovirus type 42. Arch. Virol. 94: 283-286, 1987. PubMed: 3034212 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
