| 產(chǎn)品名稱(chēng) |
Bovine herpesvirus 4 |
| 商品貨號(hào) |
B163076 |
| Classification |
Herpesviridae, Rhadinovirus |
| Deposited As |
Bovine herpesvirus |
| Agent |
Bovine herpesvirus 4 |
| Strain |
DN-599 |
| Applications |
Produces respiratory illness in calves |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Serologically distinct from infectious bovine rhinotracheitis, bovine mammilitis, malignant catarrhal fever, pseudorabies allerton and Movar type herpesvirus. Produces respiratory illness in calves. |
| Effect on Host |
CPE, refractile and rounded cells |
| Recommended Host |
BT (ATCC® CRL-1390™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are approximately 95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-4 days |
| Name of Depositor |
SB Mohanty, University of Maryland, College Park, Maryland |
| Source |
Nasal swabs from 1 ? year old steer with clinical symptoms of respiratory disease, Maryland |
| References |
Mohanty SB, et al. A new bovine herpesvirus and its effect on experimentally infected calves. Brief report. Arch. Gesamte Virusforsch. 33: 394-395, 1971. PubMed: 4107037
|
