| 產品名稱 |
10-2.16 |
| 商品貨號 |
B163533 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against I-A of the k, r, f and s haplotypes |
| Cellular Products |
immunoglobulin; monoclonal antibody; against I-A of the k, r, f and s haplotypes |
| Comments |
Animals were immunized with spleen cells from C3H mice. Spleen cells were fused with NS-1 myeloma cells. The antibody does not react with I-A of the b, d, p and q haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x exp5 10 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2b |
| Name of Depositor |
LA Herzenberg |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Oi VT, et al. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr. Top. Microbiol. Immunol. 81: 115-129, 1978. PubMed: 567555
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