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11B11 [BSF-1]

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編號:B163545

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產(chǎn)品名稱	11B11 [BSF-1]
商品貨號	B163545
Organism	Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The antibody does not cross-react with other lymphokines, and can be used to purify BSF-1 from complex mixtures.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with purified BSF-1 emulsified in complete Freund's adjuvant. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1)
Cellular Products	immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1)
Comments	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	RPMI 1640 medium with L-glutamine, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵viable cells/mL. Maintain cultures at a cell concentration between 2 x 10⁵ and 1 x 10⁶cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	70% Culture Media + 20% FBS + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Isotype	IgG1
Name of Depositor	WE Paul
Deposited As	rat (B cell); mouse (myeloma)
References	Ohara J, Paul WE. Production of a monoclonal antibody to and molecular characterization of B-cell stimulating factor-1. Nature 315: 333-336, 1985. PubMed: 2582266 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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