| 產品名稱 |
11E10 |
| 商品貨號 |
B163546 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933. The antibody reacts with the A subunit of SLT-II and does not react with B subunit. The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains. It may also be used to differentiate SLT-II from SLT-I, SLT-IIv and Shiga toxin. The 11E10 antibody differs from that of 11F11 (ATCC CRL-1908) in that it can partially neutralize SLT-IIv. |
| Derivation |
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II) |
| Comments |
Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the A subunit of SLT-II and does not react with B subunit. The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains. It may also be used to differentiate SLT-II from SLT-I, SLT-IIv and Shiga toxin. The 11E10 antibody differs from that of 11F11 (ATCC CRL-1908) in that it can partially neutralize SLT-IIv. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
AD O'Brien |
| References |
Perera LP, et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. Microbiol. 26: 2127-2131, 1988. PubMed: 3053764
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