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145-2C11

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編號:B163576

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產(chǎn)品名稱	145-2C11
商品貨號	B163576
Organism	Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Tissue	Spleen, blood
Cell Type	Hybridoma: B lymphocyte
Product Format	frozen
Morphology	Lymphoblast-like
Culture Properties	Suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor. The antibody reacts with the murine T cell receptor (CD3 - T3) complex. The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters. It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters. It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters. It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters. It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters. It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters. It reacts with all mature T cells and can both activate and inhibit T cell function.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against mouse CD3
Cellular Products	immunoglobulin; monoclonal antibody; against mouse CD3
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10⁵ cells/mL and maintain between 1 X 10⁵ and 1 X 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%
Isotype	IgG , IgG
Name of Depositor	JA Bluestone
Deposited As	hamster, Armenian (B cell); mouse (myeloma)
References	Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524 Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079

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