| 產品名稱 |
162-46.2 [Trop-2] |
| 商品貨號 |
B163593 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Choriocarcinoma |
| Derivation |
Animals were immunized with BeWo cells (ATCC CCL-98).
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the trop-2 antigen of human trophoblasts cells and choriocarcinomas |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the trop-2 antigen of human trophoblasts cells and choriocarcinomas |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
The antibody defines an antigen that is expressed on trophoblasts and choriocarcinoma cells but not on blood cells or other tumor cells.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%. |
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Isotype |
IgG1 (Igh-4a allotype) |
| Name of Depositor |
AM Stall |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529
|
