| Applications |
The antibody reacts with approximately 25 proteins containing the "alternating arginine" motif shared by RNA processing factors. It also binds to a conserved epitope ("alternating arginine") present on approximately 25 nuclear antigens, many of which are involved in pre-mRNA processing. The antibody binds to nuclear proteins in human, frog and Drosophila extracts. It can be used to determine whether a factor is involved in pre-mRNA processing; also used in isolation or depletion of RNA processing activate. |
| Comments |
Animals were immunized with purified dephosphorylated bovine SRp55 and subsequently with dSRp55-GST. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with approximately 25 proteins containing the "alternating arginine" motif shared by RNA processing factors. These include four of the family of six SR proteins (SRp20, SRp40, SRp55 and SRp75), U2AF65, U2AF35, HRH1, and U17OK. It also binds to a conserved epitope ("alternating arginine") present on approximately 25 nuclear antigens, many of which are involved in pre-mRNA processing. The epitope has been defined as containing arginine alternating with glutamate, asparate, or-phospho-serine. The antibody binds to nuclear proteins in human, frog and Drosophila extracts. It can be used to determine whether a factor is involved in pre-mRNA processing; also used in isolation or depletion of RNA processing activate. |
| References |
Neugebauer KM, et al. A conserved epitope on a subset of SR proteins defines a larger family of Pre-mRNA splicing factors. J. Cell Biol. 129: 899-908, 1995. PubMed: 7538140
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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