| 產(chǎn)品名稱 |
182-PF SK |
| 商品貨號(hào) |
B163605 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hereditary adenomatosis |
| Age |
20 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Clinical Data |
The tissue donor suffered from hereditary adenomatosis. male Caucasian 20 years |
| Comments |
The tissue donor suffered from hereditary adenomatosis. The line is capable of 10 population doublings after thawing. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days. |
| Name of Depositor |
L Kopelovich |
| Deposited As |
Homo sapiens |
| References |
Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781
Prog. Cancer Res. Ther. 13: 97-108, 1980.
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