| 產品名稱 |
20-8-4S |
| 商品貨號 |
B163638 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? C3H (B cell); BALB/c (myeloma) |
| Applications |
The antibody cross-reacts with H-2Kd, H-2Ks and H-2Kr. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against H-2 K b, D b; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against H-2 K b, D b; cytotoxic |
| Comments |
Mice were immunized with spleen cells from C3H.SW mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody cross-reacts with H-2Kd, H-2Ks and H-2Kr. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
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| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) viable cells/ml. Maintain cell density between 1 X 10 (5) and 1 X 10 (6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C |
| Isotype |
IgG2a kappa |
| Name of Depositor |
DH Sachs, K Ozato |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293
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