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20C2 [4H12:20C2.G6.C9]

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編號:B163639

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產(chǎn)品名稱	20C2 [4H12:20C2.G6.C9]
商品貨號	B163639
Organism	Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution. The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12. The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12. The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12.
Storage Conditions	liquid nitrogen vapor phase
Derivation	The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution. The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75.
Genes Expressed	immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75
Cellular Products	immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75
Comments	The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution. The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12. The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12. The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12. The 20C2 antibody does not bind the p40 or p35 subunits of IL-12. The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75.
Complete Growth Medium	Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing	Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶cells/mL. Maintain cell density between 1 x 10⁵and 1 x 10⁶viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (as cell density increases)
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG1
Name of Depositor	DH Presky
Deposited As	rat (B cell); mouse (myeloma)
Year of Origin	1991
References	Chua AO, et al. Expression cloning of a human IL-12 receptor component--a new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. 153: 128-136, 1994. PubMed: 7911493 Chizzonite R, et al. IL-12: monoclonal antibodies specific for the 40-kDa subunit block receptor binding and biologic activity on activated human lymphoblasts. J. Immunol. 147: 1548-1556, 1991. PubMed: 1715362 Chizzonite R, et al. IL-12 receptor. I. Characterization of the receptor on phytohemagglutinin-activated human lymphoblasts. J. Immunol. 148: 3117-3124, 1992. PubMed: 1578138 Desai BB, et al. IL-12 receptor. II. Distribution and regulation of receptor expression. J. Immunol. 148: 3125-3132, 1992. PubMed: 1578139 Gately MK, et alMeasurement of Human and Mouse Interleukin-12In: Gately MK, et alCurrent Protocols in ImmunologyNew York, N.Y.John Wiley and Sons1995. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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