| 產品名稱 |
2254-62.2 |
| 商品貨號 |
B163643 |
| Organism |
hamster |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
This line was derived from BHK-21(c-13) (see ATCC CCL-10). This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This line was derived from BHK-21(c-13) (see ATCC CCL-10). |
| Comments |
This line was derived from BHK-21(c-13) (see ATCC CCL-10). This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 100 nM methotrexate, 93%; dialyzed fetal bovine serum, 7%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse with fresh 0.25% trypsin, remove trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
Genentech, Inc. |
| Deposited As |
hamster |
| U.S. Patent Number |
|
| References |
Capon DJ, et al. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990
|
