| 產(chǎn)品名稱 |
236L |
| 商品貨號(hào) |
B163645 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications |
This antibody is specific for the rat subunit integrin alphaD. It may be used to inhibit inflammation at the site of a central nervous system injury. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified rat alphaD subunit. Spleen cells were fused with NS-1 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against alphaD subunit of rat beta2 (alphaDbeta2) integrin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against alphaD subunit of rat beta2 (alphaDbeta2) integrin |
| Comments |
Animals were immunized with purified rat alphaD subunit. Spleen cells were fused with NS-1 mouse myeloma cells. This antibody is specific for the rat subunit integrin alphaD. It may be used to inhibit inflammation at the site of a central nervous system injury. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated fetal bovine serum, 15%
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
Culture medium supplemented with an additional 50% heat-inactivated fetal bovine serum and 10% DMSO |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
ICOS Corporation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Gallatin WM, Van der Vieren M. Methods of inhibiting inflammation at the site of a central nervous system injury with alphaD-specific antibodies. US Patent 6,251,395 dated Jun 6 2001
Mabon PJ, et al. Inhibition of monocyte/macrophage migration to a spinal cord injury site by an antibody to the integrin alphaD: a potential new anti-inflammatory treatment. Exp. Neurol. 166: 52-64, 2000. PubMed: 11031083
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