| 產品名稱 |
2B5.3 |
| 商品貨號 |
B163672 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with G. muris trophozoite surfaces. Unlike 3C7.2 (ATCC CRL-1959), 1A3.1 (ATCC CRL-1961) and 1B1.1, the 2B5.3 antibody specifically recognizes trophozoite antigens of apparent molecular weight 31000 and 35000 on western blots. |
| Derivation |
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Giardia muris trophozoites |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Giardia muris trophozoites |
| Comments |
Animals were immunized with G. muris trophozoites. Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. The antibody reacts with G. muris trophozoite surfaces. Unlike 3C7.2 (ATCC CRL-1959), 1A3.1 (ATCC CRL-1961) and 1B1.1, the 2B5.3 antibody specifically recognizes trophozoite antigens of apparent molecular weight 31000 and 35000 on western blots. The antibody immunoprecipitates two polypeptides with apparent molecular weights of 30000. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
NCTC 109 medium, 10%;
(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);
horse serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2b; kappa light chain |
| Name of Depositor |
MF Heyworth |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009
|
