| 產品名稱 |
2C5-6 |
| 商品貨號 |
B163675 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications |
It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against cholesterol |
| Cellular Products |
immunoglobulin; monoclonal antibody; against cholesterol |
| Comments |
Animals were immunized with liposomes containing high amounts of cholesterol and lipid A as an adjuvant. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody detects cholesterol in liposomes containing 71% cholesterol but not with liposomes containing 43% cholesterol. It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay. The antibody is capable of detecting cholesterol in situ in the membranes of skeletal muscle sections. A feeder layer of mouse peritoneal macrophages or mouse fibroblasts (see ATCC 56-X, irradiated STO cells) is require to propagate this cell line. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM |
| Name of Depositor |
G Schwartz |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Clarke MS, et al. In situ localization of cholesterol in skeletal muscle by use of a monoclonal antibody. J. Appl. Physiol. 89: 731-741, 2000. PubMed: 10926660
Swartz GM Jr., et al. Antibodies to cholesterol. Proc. Natl. Acad. Sci. USA 85: 1902-1906, 1988. PubMed: 3162316
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