| 產品名稱 |
2D-7 |
| 商品貨號 |
B163679 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
|
| Applications |
The antibody reacts with a major epitope on human CENP-B. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Clinical Data |
The binding of the 2D-7 antibody is at least partially blocked by sera from patients with anticentromere antibodies. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human centromere protein B (CENP-B) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human centromere protein B (CENP-B) |
| Comments |
Animals were immunized with human CENP-B peptide. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a major epitope on human CENP-B. The binding of the 2D-7 antibody is at least partially blocked by sera from patients with anticentromere antibodies. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Johns Hopkins University |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Earnshaw W, et al. Cloned human centromere autoantigen. US Patent 5,196,307 dated Mar 23 1993
|
