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32.2

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編號:B163702

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產(chǎn)品名稱	32.2
商品貨號	B163702
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain	Strain:? BALB/c (B cell); BALB/c (myeloma)
Applications	The antibody is specific for the 72000 dalton, high affinity Fc receptor on human monocytes.
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	Animals were immunized with partially purified FcR from U937 cells. Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against human monocyte Fc receptor (high affinity, FcRI), CD64
Cellular Products	immunoglobulin; monoclonal antibody; against human monocyte Fc receptor (high affinity, FcRI), CD64
Comments	Animals were immunized with partially purified FcR from U937 cells. Spleen cells were fused with NS-1 myeloma cells. The antibody is specific for the 72000 dalton, high affinity Fc receptor on human monocytes. Binding of the antibody is not inhibited by human IgG, and the antibody does not block human IgG binding to the receptor.
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 6 mM HEPES and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype	IgG1
Name of Depositor	Trustees of Dartmouth College
Deposited As	mouse (B cell); mouse (myeloma)
U.S. Patent Number	4,954,617
References	Graziano RF, Fanger MW. FcgammaRI and FcgammaRII on monocytes and granulocytes are cytotoxic trigger molecules for tumor cells. J. Immunol. 139: 3536-3541, 1987. PubMed: 2960735 Petroni KC, et al. Modulation of human polymorphonuclear leukocyte IgG Fc receptors and Fc receptor-mediated functions by IFN-gamma and glucocorticoids. J. Immunol. 140: 3467-3472, 1988. PubMed: 2966197 Fanger MW, et al. Monoclonal antibodies to FC receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 4,954,617 dated Sep 4 1990 Fanger MW, et al. Cytotoxicity mediated by human Fc receptors for IgG. Immunol. Today 10: 92-99, 1989. PubMed: 2525910 Shen L, et al. Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J. Immunol. 139: 534-538, 1987. PubMed: 2955043 Shen L, et al. Heteroantibody-mediated cytotoxicity: antibody to the high affinity Fc receptor for IgG mediates cytotoxicity by human monocytes that is enhanced by interferon-gamma and is not blocked by human IgG. J. Immunol. 137: 3378-3382, 1986. PubMed: 2946759 Anderson CL, et al. Monoclonal antibodies to Fc receptors for IgG on human mononuclear phagocytes. Antibody characterization and induction of superoxide production in a monocyte cell line. J. Biol. Chem. 261: 12856-12864, 1986. PubMed: 3017990 Ball ED, Fanger MW. Bispecific molecules for use in inducing antibody dependent effector cell-mediated cytotoxicity. US Patent 5,833,985 dated Nov 10 1998 Tempest PR, et al. Humanized antibodies to Fc receptors for immunoglobulin G on human mononuclear phagocytes. US Patent 6,500,931 dated Dec 31 2002

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