| 產品名稱 |
34-4-20S |
| 商品貨號 |
B163711 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody cross-reacts with H-2Kb, H-2Kq and H-2r. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against H-2 D d; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against H-2 D d; cytotoxic |
| Comments |
Animals were immunized with spleen cells from BDF1 mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody cross-reacts with H-2Kb, H-2Kq and H-2r. Does not react with H-2Kk. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
DH Sachs, K Ozato |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Ozato K, et al. Monoclonal antibodies to mouse MHC antigens. IV. A series of hybridoma clones producing anti-H-2d antibodies and an examination of expression of H-2d antigens on the surface of these cells. Transplantation 34: 113-118, 1982. PubMed: 7135466
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