| 產品名稱 |
35.1 |
| 商品貨號 |
B163715 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the human T cell surface protein Tp50 (CD2). |
| Derivation |
Spleen cells were fused with NSI/1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD2 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD2 |
| Comments |
Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA). Spleen cells were fused with NSI/1 myeloma cells. The antibody reacts with the human T cell surface protein Tp50 (CD2). The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity. |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
PJ Martin |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. PubMed: 6190907
|
