| 產品名稱 |
356-1 |
| 商品貨號 |
B163716 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a moderately conserved epitope on the JVB strain of CB4. The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit. Neutralizes many human isolates of CB4. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); also reacts with heart muscle (myocardium); neutralizing |
| Cellular Products |
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); also reacts with heart muscle (myocardium); neutralizing |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 144). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a moderately conserved epitope on the JVB strain of CB4. The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit. Neutralizes many human isolates of CB4. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium (containing 1 mM sodium
pyruvate) with 4.5 g/L glucose, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
BS Prabhakar |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593
Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617
Ungar BL, et al. Enzyme immunoassay detection of immunoglobulin M and G antibodies to Cryptosporidium in immunocompetent and immunocompromised persons. J. Infect. Dis. 153: 570-578, 1986. PubMed: 3950440
Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930
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