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3C9-D11-H11

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編號:B163734

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產品名稱	3C9-D11-H11
商品貨號	B163734
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	Tested and found negative for ectromelia virus (mousepox).
Storage Conditions	liquid nitrogen vapor phase
Derivation	Mice were immunized with purified PPV. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Cellular Products	immunoglobulin; monoclonal antibody; against porcine parvovirus (PPV)
Tumorigenic	Yes
Effects	Yes, in BALB/c mice
Comments	Mice were immunized with purified PPV. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody has both virus neutralization and hemagglutinating activities. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
Subculturing	Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: Two to three times weekly
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	IgG1
Name of Depositor	RA Van Deusen
Deposited As	mouse (B cell); mouse (myeloma)
References	8th Proc. Intl. Pig. Vet. Soc. Gent, Belgium: 1984. Katz JB, van Deusen RA. Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system. J. Virol. Methods 12: 193-198, 1985. PubMed: 3009510 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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