| 產品名稱 |
3G5 |
| 商品貨號 |
B163738 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Insulinoma |
| Applications |
The antibody reacts with an acetylated ganglioside common to pancreatic islet cells, neurons, adrenal medulla, thyroid follicle renal glomerulus, human T lymphocytes and a majority of cells in the pituitary. The antibody reacts with an O-acetylated disialoganglioside present on a wide variety of endocrine and neuronal tissues. The 3G5 antigen is expressed on microvascular pericyte plasma membranes, and is useful as a marker of pericytes in vitro. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Derivation |
Spleen cells were fused with myeloma cells. |
| Genes Expressed |
Tested and found negative for ectromelia virus (mousepox). immunoglobulin; monoclonal antibody; against a ganglioside associated with endocrine cells (pancreatic islet, adrenal medulla, pituitary, thyroid), human T lymphocytes and neuronal cells,Increasing expression of the 3G5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F). |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a ganglioside associated with endocrine cells (pancreatic islet, adrenal medulla, pituitary, thyroid), human T lymphocytes and neuronal cells |
| Comments |
Animals were immunized with fetal rat brain. Spleen cells were fused with myeloma cells. The antibody reacts with an acetylated ganglioside common to pancreatic islet cells, neurons, adrenal medulla, thyroid follicle renal glomerulus, human T lymphocytes and a majority of cells in the pituitary. The antibody reacts with an O-acetylated disialoganglioside present on a wide variety of endocrine and neuronal tissues. In addition, the antibody recognizes an antigen on human T lymphocytes that defines a subset of T cells that increases with age. Increasing expression of the 3G5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F). The 3G5 antigen is expressed on microvascular pericyte plasma membranes, and is useful as a marker of pericytes in vitro. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM kappa , IgM; kappa light chain |
| Name of Depositor |
GS Eisenbarth, R Akeson, RC Nayak |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Rabinowe SL, et al. Aging in man: Linear increase of a novel T cell subset defined by antiganglioside monoclonal antibody 3G5. J. Exp. Med. 165: 1436-1441, 1987. PubMed: 3494809
Powers AC, et al. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338-1343, 1984. PubMed: 6368201
Nayak RC, et al. A monoclonal antibody (3G5)-defined ganglioside antigen is expressed on the cell surface of microvascular pericytes. J. Exp. Med. 167: 1003-1015, 1988. PubMed: 3351433
Bartholomeusz RK, et al. Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. Endocrinology 124: 2680-2685, 1989. PubMed: 2541995
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