| 產品名稱 | 3T3-Swiss albino |
|---|---|
| 商品貨號 | B163740 |
| Organism | Mus musculus, mouse |
| Tissue | embryo |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | embryo |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | This is a hypertriploid mouse cell line. The modal chromosome number was 68 occurring in 30% of cells. The rate of cells with higher ploidies was 2.4%. |
| Images |  |
| Derivation | The 3T3 cell line was established by G. Todaro and H. Green in 1962 from disaggregated Swiss mouse embryos. |
| Genes Expressed | Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway. Ref   Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 |
| Cellular Products | Lysophosphatidylcholine (lyso-PC) induces AP-1 activity and c-jun N-terminal kinase activity (JNK1) by a protein kinase C-independent pathway
Ref Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 |
| Virus Susceptibility | polyomavirus
SV40 virus |
| Comments | The cells are contact inhibited.
A confluent monolayer yields 40000 cells/cm2.
Tested and found negative for ectromelia virus (mousepox).
The cells should be grown in plastic flasks, they do not grow well on some types of glass surfaces.
A saturation density of approximately 50000 cells/cm2 can be reached. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
|
| Subculturing | Never allow culture to become completely confluent. Subculture when 80% confluent or less. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: Twice per week |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C |
| Population Doubling Time | 18 hrs |
| Name of Depositor | H Green |
| Deposited As | Mus musculus |
| Year of Origin | 1962 |
| References | Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244 Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596 Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482 Todaro GJ, et al. Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40. Virology 27: 179-185, 1965. PubMed: 4284655 Todaro GJ, et al. Transformation of properties of an established cell line by SV40 and polyoma virus. Proc. Natl. Acad. Sci. USA 51: 66-73, 1964. PubMed: 14104605 Fang X, et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 Chen ST, et al. Generation of packaging cell lines for pseudotyped retroviral vectors of the G protein of vesicular stomatitiis virus by using a modified tetracycline inducible system. Proc. Natl. Acad. Sci. USA 93: 10057-10062, 1996. PubMed: 8816750 Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326 Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936 |
| Cross References | Nucleotide (GenBank) : X85983 M.musculus mRNA for carnitine acetyltransferase. Nucleotide (GenBank) : U85711 Mus musculus phospholipase C delta-1 mRNA, complete cds. Nucleotide (GenBank) : U85713 Mus musculus phospholipase C-beta-1b mRNA, complete cds. Nucleotide (GenBank) : U85714 Mus musculus phospholipase C-beta-1b' mRNA, complete cds. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
