| 產品名稱 |
4A6 |
| 商品貨號 |
B163758 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an NEM sensitive fusion protein required for vesicular transport through the golgi stack. The antibody should not be frozen, and should be used in buffers containing at least 65 mM KCl to avoid precipitation. |
| Derivation |
Animals were immunized with purified N-ethylmaleimide (NEM) sensitive factor. Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against golgi vesicular transport protein |
| Cellular Products |
immunoglobulin; monoclonal antibody; against golgi vesicular transport protein |
| Comments |
Animals were immunized with purified N-ethylmaleimide (NEM) sensitive factor. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with an NEM sensitive fusion protein required for vesicular transport through the golgi stack. The antibody should not be frozen, and should be used in buffers containing at least 65 mM KCl to avoid precipitation. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgM |
| Name of Depositor |
JE Rothman |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
Block MR, et al. Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. Proc. Natl. Acad. Sci. USA 85: 7852-7856, 1988. PubMed: 3186695
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