| 產(chǎn)品名稱 |
4D11 |
| 商品貨號 |
B163765 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells. This subset (LGL-1+/NK-1.1+) functions in NK type lysis, but lacks LAK (lymphokine activated killing) activity against P815 and L5178Y tumor target cells. The antigen recognized by 4D11 appears to be nonpolymorphic, and is present in all mouse strains tested. |
| Derivation |
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against an antigen on mouse NK cells (LGL-1) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against an antigen on mouse NK cells (LGL-1) |
| Comments |
Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells. Spleen cells were fused with NS-1 myeloma cells. The antibody detects a major (approximately 50%) subset of mouse NK cells. This subset (LGL-1+/NK-1.1+) functions in NK type lysis, but lacks LAK (lymphokine activated killing) activity against P815 and L5178Y tumor target cells. The antigen recognized by 4D11 appears to be nonpolymorphic, and is present in all mouse strains tested. |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
LH Mason |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Mason LH, et al. Natural killer (NK) cell subsets in the mouse. NK-1.1+/LGL-1+ cells restricted to lysing NK targets, whereas NK-1.1+/LGL-1- cells generate lymphokine-activated killer cells. J. Immunol. 145: 751-759, 1990. PubMed: 2365999
Mason L, et al. LGL-1: a non-polymorphic antigen expressed on a major population of mouse natural killer cells. J. Immunol. 140: 4403-4412, 1988. PubMed: 2453582
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