| 產品名稱 |
4D12 |
| 商品貨號 |
B163766 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an HLA determinant that includes specificities within, but not identical to, the B5 cross-reactive group. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA B5 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA B5 |
| Comments |
Animals were immunized with the HUT 78 Sezary cutaneous T cell line. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody reacts with an HLA determinant that includes specificities within, but not identical to, the B5 cross-reactive group. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
heat-inactivated fetal bovine serum, 20%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
BF Haynes |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Haynes BF, et al. Description of monoclonal antibody defining an HLA allotypic determinant that includes specificities within the B5 cross-reacting group. Hum. Immunol. 4: 273-285, 1982. PubMed: 6181035
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