| 產品名稱 |
4D9D4 |
| 商品貨號 |
B163767 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with Drosophila invected protein produced in E. coli. The antibody cross-reacts with engrailed protein of Drosophila, grasshopper, leech, chicken, zebra fish, lobster and crayfish. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with Drosophila invected protein produced in E. coli. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the invected and engrailed proteins of Drosophila melanogaster |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the invected and engrailed proteins of Drosophila melanogaster |
| Comments |
Animals were immunized with Drosophila invected protein produced in E. coli. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with engrailed protein of Drosophila, grasshopper, leech, chicken, zebra fish, lobster and crayfish. It does not recognize mouse (En-1) or sea urchin engrailed proteins. The epitope recognized by the antibody is within the homeodomain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
TB Kornberg |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Patel NH, et al. Expression of engrailed proteins in arthropods, annelids, and chordates. Cell 58: 955-968, 1989. PubMed: 2570637
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