| 產品名稱 |
51.1 |
| 商品貨號 |
B163775 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8). The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey. |
| Derivation |
Spleen cells were fused with NSI/1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD8 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD8 |
| Comments |
Animals were immunized with human peripheral blood T lymphocytes. Spleen cells were fused with NSI/1 myeloma cells. The antibody reacts with an antigen associated with human cytotoxic/suppressor T cells (CD8). The antibody strongly inhibits cytotoxic T lymphocyte function and cross-reacts with peripheral blood T cells from chimpanzee, orangutan, gibbon, baboon, macaque, capuchin monkey, squirrel monkey and spider monkey. |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate suppelmented with 50 ?M 2-mercaptoethanol, 90%; fetal bovine serum,
10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
PJ Martin |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Martin PJ, et al. Epitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32. J. Immunol. 132: 759-765, 1984. PubMed: 6197474
|
