| 產品名稱 |
55-2 |
| 商品貨號 |
B163783 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Suzanne Epstein that reacts with the gp120 of HIV-1. In ELISA the antibody reacts with rgp120 IIIB, but not with gp120 MN. It binds to the C2 region (positions 254 - 274) gp120 synthetic peptide used to map antibody reactivity, and was not able to cross block any other antibodies in the panel. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. 55-2 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV-1) gp120 |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were sequentially immunized with recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. 55-2 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1. In ELISA the antibody reacts with rgp120 IIIB, but not with gp120 MN. It binds to the C2 region (positions 254 - 274) gp120 synthetic peptide used to map antibody reactivity, and was not able to cross block any other antibodies in the panel. |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
SL Epstein |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Reeves JP, et al. Mouse monoclonal antibodies to human immunodeficiency virus glycoprotein 120 generated by repeated immunization with glycoprotein 120 from a single isolate, or by sequential immunization with glycoprotein 120 from three isolates. Hybridoma 14: 235-242, 1995. PubMed: 7590785
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